If you are running the cloning process on the PC you want to clone, you will need to either use the bundled startup software or boot using a live image to avoid booting into Windows so that the disk may be copied in its entirety. If you're comfortable with a limited interface or command-line solution, Clonezilla or even dd are standard utilities for this. User friendly products include Paragon, EaseUS, and a host of others. This is normally done via a harddrive cloning utility. There are several paid and free solutions to clone a hard drive to a virtual machine. I'm not sure ISO is the right format you're looking for, as that is used for optical discs.
